Extracytoplasmic proteins of Mycobacterium tuberculosis – mature secreted proteins often start with aspartic acid and proline

Heterologous Expression, Purification, and Characterization of the HspX, Ppe44, and EsxV Proteins of Mycobacterium tuberculosis

Background: Subunit vaccines are appropriate vaccine candidates for the prevention of some infections. In this study, three immunogenic proteins of Mycobacterium tuberculosis, including HspX, Ppe44, and EsxV as a new construction, were expressed alone and as a fusion protein to develop a new vaccine candidate against tuberculosis infection. Methods: To make the fusion protein, the three genes ...

The Identification of proteins present in culture filtrare of Mycobacterium tuberculosis DT and comparison with human tuberculin proteins by electrophoretic methods

Abstract Background and objectives: Tuberculin is the proteins existed in tuberculosis culture medium which precipitated by trichloroacetic acid (TCA) or ammonium sulfate. Tuberculin is used for diagnosis of Tuberculosis. The aim of this study is to compare the human tuberculin produced by Razi Institute and Mycobacterium tuberculosis Culture Filtrate Protein. Material and Methods: Initially By...

Computational Prediction and Experimental Assessment of Secreted/Surface Proteins from Mycobacterium tuberculosis H37Rv

The mycobacterial cell envelope has been implicated in the pathogenicity of tuberculosis and therefore has been a prime target for the identification and characterization of surface proteins with potential application in drug and vaccine development. In this study, the genome of Mycobacterium tuberculosis H37Rv was screened using Machine Learning tools that included feature-based predictors, ge...

Secreted Mycobacterium tuberculosis Rv3654c and Rv3655c Proteins Participate in the Suppression of Macrophage Apoptosis

BACKGROUND Inhibition of macrophage apoptosis by Mycobacterium tuberculosis has been proposed as one of the virulence mechanisms whereby the pathogen avoids the host defense. The mechanisms by which M. tuberculosis H37Rv strain suppress apoptosis and escapes human macrophage killing was investigated. METHODOLOGY/PRINCIPAL FINDINGS The screening of a transposon mutant bank identified several m...

Mycobacterium tuberculosis Vaccines Expressing Secreted Proteins of Differential Protective Efficacy of DNA